radioactive probes are not required in. option .1. RAPD. option .2. RFLP. option 3.. AFLP. option. 4 none of these
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Answer:
option 4
Explanation:
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Question:
Which of the following DNA markers does not required radioactive probes?
1. RAPD
2. RFLP
3. AFLP
4. None of the above
Answer:
In RAPD radioactive probes are not required.
Explanation:
- RFLP is the Restriction Fragment Length Polymorphism and it is the widely used hybridization-based molecular marker. As it is the hybridization-based molecular marker, so in most of the RFLPs radioactive probes are required.
- RAPD stands for Random Amplification of Polymorphic DNA. It is a type of PCR (Polymerase Chain Reaction), but the segments of DNA that are amplified are random and radioactive probes are not required in this dominant marker.
- To overcome the limitation of reproducibility associated with RAPD, AFLP (Amplified Fragment length Polymorphism) technology was developed. It is a dominant marker. AFLPs can be either detected with radioactive label probe or with silver staining.
Hence, the correct option is 1.
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