Biology, asked by Jananii5383, 1 year ago

Real time control primer for arabidopsis

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Answered by Anonymous
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Three primer sets designed for use as internal controls in PCR-based Arabidopsis gene expression analysis. All three primer sets have been functionally validated and optimized by RT-PCR analysis against major Arabidopsis tissues for constitutive expression and specific amplification (see Figures 1 & 2). Further confirmation of those primers, as internal control for data normalization, was conducted in quantitative RT-PCR reactions for pathogen-inducible Arabidopsis genes.

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