Recovery percent meaning in perform accuracy in method validation
Answers
Answer:
The accuracy of an analytical method is the degree of agreement of test results generated by the method to the true value. Accuracy is measured by spiking the sample matrix of interest with a known concentration of analyte standard and analyzing the sample using the “method being validated.”
Answer:
It is not a high recovery but rather a recovery near 100 % (e.g. 80-110 %) that gives confidence. One important aspect of recoveries near 100% is that it is an indication of an almost complete mass balance of the recovery standard. It basically tells you that you know what you are doing (at least with the recovery standard) in terms of (1) complete extraction, (2) minimal losses, (3) good alignment between spiking and calibration solution and (4) also the analytical system.
Recovery corrections are another aspect. Recoveries near 100 % are particularly important when recovery corrections would be associated with large errors. When you use only one recovery standard for a multianalyte analyses, you better reach high recoveries since it will basically be impossible to make good recovery corrections. To the contrary, when working with 13C labeled analogs as internal standards, you can have excellent accuracy and precision even when recoveries are at 50%.