Question

Researchers are trying to make a genetically modified (gm) pig that expresses bovine (cow) growth hormone to make it grow bigger. They isolated the bovine growth hormone gene, cut it and a plasmid with the same restriction enzyme, and mixed them together. They inserted the mixture into pig zygotes, but only normal pigs, not gm pigs, grew. What did they likely do wrong?

Answer 1

Answer:

they didnt join the cut plasmid and the bovine growth hormone gene with DNA Ligase. this will not embed the desired gene into the plasmid and hence into the pig zygote.

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