Question

Restriction endonuclease are useful in

Answer 1

Answer:

A restriction endonuclease is an enzyme that cuts the DNA molecule at, or near to, a specific nucleotide sequence to produce discrete DNA fragments that can be separated by gel electrophoresis. From: Medical Microbiology (Eighteenth Edition), 2012.

Explanation:

hope you understand

Answer 2

Answer

• Each restriction endonuclease have a specific point by recognising a specific sequence of four to eight nucleotides.

• It is known as recognition sequence or specific sites for palindromic sequence.

• The pallindrome in DNA is a sequence of base pairs that reads same on the two strands when orientation of reading is kept the same.

• The DNA having modified sequences cannot be recognised and cut by restriction enzymes.

• This is how a bacterium protects its own chromosomal DNA from cleavage by these restriction enzymes.