Question

Restriction enzyme ‘Eco RI’ nicks at one of the
following sites on DNA
(a) 5'G↓AATTC3' (b) 5'GG­↑CC3'
(c) 5'A↓AGCTT 3' (d) None above

Answer 1

$\huge\bold\red{Hey!}$

Restriction enzyme ‘Eco RI’ nicks at 5'A↓AGCTT 3'.

Answer 2

Restriction enzyme ‘Eco RI’ nicks at one of the following sites on DNA- 5'G↓AATTC3'.

* It is a restriction endonuclease which has been isolated from a particular strain of Escherichia coli.

* It is a type II restriction endonuclease that recognises specific palindromic sequences of about 4 to 8 base pairs in length.

* The site for cutting is also within the given sequence. After the cut, it produces two complementary ends.

* Other examples of type II restriction endonucleases are Hind III, BamH1, etc.