Restriction enzymes present in the cloning site of a vector should not have more than one recognition site. Comment.
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Presence of more than one recognition site in a vector will cause the restriction enzyme to act on more than one place and fragment the vector.
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Restriction enzymes should not have more than one site of. Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment. Enzymes should not have more than one site of action.
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