Question

Restriction enzymes present in the cloning site of a vector should not have more than one recognition site. Comment.

Answer 1

Presence of more than one recognition site in a vector will cause the restriction enzyme to act on more than one place and fragment the vector.

Answer 2

Restriction enzymes should not have more than one site of. Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment. Enzymes should not have more than one site of action.