Reverse phase chromatography procedure application
Answers
Reversed-phase chromatography employs a polar (aqueous) mobile phase. As a result, hydrophobic molecules in the polar mobile phase tend to adsorb to the hydrophobic stationary phase, and hydrophilic molecules in the mobile phase will pass through the column and are eluted first.
The term reversed-phase describes the chromatography mode that is just the opposite of normal phase, namely the use of a polar mobile phase and a non-polar [hydrophobic] stationary phase. ... A C18–bonded silica [sometimes called ODS] is the most popular type of reversed-phase HPLC packing.
Application: Micro purification of protein fragments for sequencing.
The process scale purification of recombinant protein products.
Hope this helps...
Answer:
Application:
micro purification of protein fragments for sequencing
the process scale
purification of recombinate protein products.
I hope we will help you with me ☺️