Rima used common salt instead of soda lime in the experiment to show requirement of carbon dioxide in the process of photosynthesis.What do you think will be result of the experiment
Answers
Experiment to prove the necessity of carbon dioxide in photosynthesis - definition. Insert a part of the leaf of a destarched plant into a conical flask containing potassium hydroxide. Potassium hydroxide solution absorbs the carbon dioxide gas from the air present in the glass bottle. Leave the plant sunlight.
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Answer:
Experiments to show the factors required in photosynthesis (2) - light and carbon dioxide
These experiments start with destarched potted plants - Geranium (Pelargonium) is often used.
These are then subjected to various treatments, left in the light for a few days then subjected to decolourising procedures and then finally tested for the presence of starch.
Destarching the plant:
The plant is placed in a dark place, e.g. a cupboard for 24 or 48 hours. It is essential that the plant is watered beforehand, and not forgotten about!
This is so as to force the plant to use its reserve of starch, and not allow its replacement by photosynthesis.
Before proceeding to the next stage, a leaf is removed and checked for the presence of starch (See under the section "Testing a leaf for starch").
If no starch is present, then it is possible to proceed to the next stage. If it is, then it must be returned to the dark.
Treatments
A The effect of light
Part of the leaf may be covered with dark paper or card - possibly with perforations - and held in place with paper clips.
stage 1
Alternatively, an envelope of aluminium foil (with a distinctively shaped hole in the centre) may be placed over both sides of the leaf, and the edges of the hole pinched in a little so that light only reaches the exposed part of the leaf.
Another method involves using a pair of thin cork or discs which are used to sandwich the leaf, held in place by a pin.Click for a photo of results
It is also possible to attach a photographic negative to a leaf using a special holder.
B The effect of carbon dioxide
Leaves of the plant are enclosed within glass flasks, supported at an angle by stands and clamps (250/350ml wide-mouthed flasks will usually do). Each flask will contain small amounts of various liquids, which should not be allowed to touch the leaves.
CorrosB1 Sodium hydroxide solution or potassium hydroxide solution - CAUTION - CORROSIVE!
B2 Sodium hydrogencarbonate solution or simply carbonated water
B3 water
B4 no liquid
2flasksAfter the insertion of the leaf, 2 wads of absorbent cotton wool are placed in the neck of the flask, one above and one below the petiole (leaf stalk). These are then saturated with water (from a washbottle). This may need to be repeated a couple of times a day.
Alternatively, split corks or bungs can be used, together with lots of petroleum jelly (messy!).
The plant must be kept well watered and exposed to good light.
After a couple of days or more, the leaves are removed for testing. It may be useful to mark them with cuts to identify them later
Explanation:
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