Question

Ritika is making a temporary mount of onion peel. What precautions should be taken to avoid the entry of air bubbles on the slide? *

(a) Coverslip should be dropped on to the peel from a certain height.

(b) Peel should be allowed to fold over itself once or twice.

(c) Coverslip should be gently dropped over the peel.

(d) Peel should be immersed in stain for over an hour.

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Answer 1

(c)

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Answer 2

Answer:

The correct answer is option (c) Coverslip should be gently dropped over the peel.

Explanation:

The procedure of making temporary mount on onion peel:

• Take a piece of onion and bend it to remove the transparent membranous structure called onion epidermal peel.
• Remove the peel from its inner side with the help of forcep.
• Place the peel in water in a watch glass.
• Add a many drops of stain safranin, to the watch glass containing the peel for staining.
• Now, wash the splint peel with water and transfer it on to a clean slide with the help of encounter.
• Remove Redundant water from the slide girding the peel with the help of blotting paper.
• To this slide, add a drop of glycerine over the peel and place the coverslip in a manner to avoid entry of air bubbles.
• Soak down the redundant glycerine with blotting paper.
• Examine slide under the microscope.

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