Question

Role of glucose in solution 1 of plasmid isolation

Answer 1

The purpose of this step is to increase the starting volume of cells so that more plasmid DNA can be isolated per prep. Glucose is added to increase the osmotic pressure outside the cells. Tris is a buffering agent used to maintain a constant pH ( = 8.0)

Explanation:

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Answer 2

Answer:

contains glucose, Tris, and EDTA. Glucose is added to increase the osmotic pressure outside the cells. Tris is a buffering agent used to maintain a constant pH ( = 8.0). EDTA protects the DNA from degradative enzymes (called DNAses); EDTA binds divalent cations that are necessary for DNAse activity.

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