Role of interferon gamma in type 2 dm with foot ulcer
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zhe to the meeting and then we will be in the office tomorrow to get it back on my card and then I have addmiti I am writing okay be safe I have
Vijaykodipally9259, I have no idea whether you have any prerequisite knowledge about this subject, but I might be wrong considering the depth of your question.
Fibroblasts from scaffold-based three-dimensional human cultures have been demonstrated to colonize ulcer wound beds and persist.
Studies in monolayer cultures showed that fibroblasts expressed CD40 at about 10% of the surface density seen in umbilical vein endothelial cells, whereas HLA-DR was undetectable. In these cultures, both molecules were induced by γ-interferon.
In scaffold-based three-dimensional cultures, however, a majority of the fibroblasts showed little induction of CD40 and HLA-DR in response to γ-interferon, although HLA class I expression was increased. Fibroblasts re-isolated from the three-dimensional cultures and cultured in monolayers recovered HLA-DR induction in response to γ-interferon. Fibroblasts cultured in an alternative three-dimensional system using collagen gels showed CD40 and HLA-DR induction by γ-interferon in the same manner as monolayer cultures. Comparison of phosphorylation of signal transducer and activator of transcription 1 on tyrosine-701 showed it to be similar in monolayer and three-dimensional culture.
Thus, interaction of fibroblasts with the fibroblast-derived extracellular matrix is an important modulator of γ-interferon responsiveness and this interaction may play a role in the low immunogenicity of allogeneic fibroblasts grown on scaffolds.