Role of karyotyping in plant species identification
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Karyotypes of 27 species (2n=10, 12 and 14) belonging to the subgenus Vicia have been inserted as the training set for Linear Discriminant Analysis (a statistic procedure that uses the variables of known groups or species to classify-identify new unknown groups or species using the same variables). Many of the accessions inserted in the identifi er as unknown (40 new accessions analysed) have been identifi ed correctly. Besides, identifi -cation mistakes or species attribution have been highlighted. In fact, in some cases, the recorded karyotype for an analysed accession could not be corresponded to the literature data for that species of the related accession stored in the germplasm bank. In these cases Linear Discriminant Analysis (Identifi er) has given a precise indication of the similarity of the analysed karyotype in the Data Base (Training set), with the specie having much more similar chromosomes. This type of identifi cation, realised only with the karyotype data, appears to be the fi rst example of karyotype used as a tool to identify plant species, using multivariate analysis techniques.
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