Question

Role of mgcl2 in competent cell preparation

Answer 1

Explanation:

the addition of calcium chloride to cell suspention promote the bidding onplasmid DNA lipopolysaccharides LPS. the positive charge calcium ions attract negative charge DNA backbone and nagatively charge group in LPS inner core .

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Answer 2

Answer:

Explanation:

MgCl2 acts in the same way as does CaCl2. It induces the ability of the cells to take up DNA by altering the permeability of the membranes. The negatively charged incoming DNA is repelled by the negatively charged macromolecules present on the bacterium’s outer surface which is neutralized by the addition of MgCl2 to neutralize the unfavorable  interactions.