Role of micropropagation and tissue culture techniques
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Plant tissue culture is the technique of maintaining and growing aseptically plant cells, tissues or organs on artificial medium in test tube under controlled environmental conditions. The part which is cultured is called explant, i.e., any part of a plant taken out and grown in a test tube, under sterile conditions in special nutrient media. Plant tissue culture relies on the fact that the plants have a capacity to generate a whole plant from any cell/explant (cellular totipotency). Single cells, plant cells without cell walls (protoplasts), pieces of leaves, stems or roots, embryo, anther can be used to generate a new plant.
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Micropropagation is the tissue culture technique used for rapid vegetative multiplication of ornamental plants and fruit trees. This method of tissue culture produces several plants. Each of these plants will be genetically identical to the original plant from where they were grown.
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