Question

Role of na2co3 in media preparation for animal cell culture

Answer 1

The addition of sodium bicarbonate (NaHCO3) causes a high buffer capacity in the cell culture medium and keeps the pH-value in the physiological area during cultivation. For cells that grow rapidly, only media with a high bicarbonate content are apt or a corresponding addition of sodium bicarbonate to bicarbonate-free media is necessary, if the cell culture is performed in a CO2 incubator. The required CO2 concentration is related to the desired pH-value and the NaHCO3 concentration of the medium.

Answer 2

The addition of sodium bicarbonate (NaHCO3) causes a high buffer capacity in the cell culture medium and keeps the pH-value in the physiological area during cultivation.

For cells that grow rapidly, only media with a high bicarbonate content are apt or a corresponding addition