Question

Role of phenol chloroform isoamyl alcohol in plasmid isolation

Answer 1

This is thought to aid in the extraction, as this dissolved phenol helps denature proteins while they are still in the aqueous solution. ... Phenol/Chloroform – This is a mixture of buffer-saturated phenol and chloroform, usually close to 1:1 for DNA purification with other ratios sometimes used for RNA purification.