Screening and isolation of tannase producing bacteria
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Use of natural tannin in the screening of tannase producing microbes is really promising. The present work describes about the possibility and integrity of the newly formulated method over the previously reported methods. Tannin isolated from Terminalia belerica Roxb. (Bahera) was used to differentiate between tanninolytic and nontanninolytic microbes. The method is simple, sensitive and superior for the rapid screening and isolation of tannase-producing microbes.
Keywords: natural tannin, Bahera, tannase, plate screening, enteric yeast
Tannin acyl hydrolase (E.C: 3.1.1.20), generally known as tannase, hydrolyzes tannin (a plant constituent) to yield glucose and gallic acid. A diverse group of bacteria and fungi can synthesize tannase as on required basis (2, 9, 10, 13). At present, tannase is used in different industrial sectors, mainly, for preparation of gallic acid, instant tea, acron wine, coffee flavoured soft drinks, high-grade leather tannin, clarification of beer, fruit juices, etc. (3, 5, 6). Gallic acid, a hydrolytic product of tannin, has different uses like preparation of trimethoprim (bacteriostatic antibiotic), pyrogallol and propyl gallate (14). Recently, it has also been reported that tannase producing bacteria are associated with advanced stage in the colon cancer, allocating the possibility of bacterial tannase as a biomarker for colon cancer (17). In respect of its usefulness, potent tannase producers are fewer as reported throughout the world (20).
Keywords: natural tannin, Bahera, tannase, plate screening, enteric yeast
Tannin acyl hydrolase (E.C: 3.1.1.20), generally known as tannase, hydrolyzes tannin (a plant constituent) to yield glucose and gallic acid. A diverse group of bacteria and fungi can synthesize tannase as on required basis (2, 9, 10, 13). At present, tannase is used in different industrial sectors, mainly, for preparation of gallic acid, instant tea, acron wine, coffee flavoured soft drinks, high-grade leather tannin, clarification of beer, fruit juices, etc. (3, 5, 6). Gallic acid, a hydrolytic product of tannin, has different uses like preparation of trimethoprim (bacteriostatic antibiotic), pyrogallol and propyl gallate (14). Recently, it has also been reported that tannase producing bacteria are associated with advanced stage in the colon cancer, allocating the possibility of bacterial tannase as a biomarker for colon cancer (17). In respect of its usefulness, potent tannase producers are fewer as reported throughout the world (20).
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