Select the 2 atoms that act as nucleophiles in the acylation phase. Note: as shown below, the nucleophilic atoms might be weak nucleophiles, but they become strong in the process of catalysis.
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The 2 atoms acts as the Electrophiles in the acylation phase and it mainly the first step of chymotrypsin mechanism.
The alkoxide ion on Ser195 attacks the carbonyl carbon of peptide substrate.
Mechanisms for the 3 enzymes: chymotrypsin, hexokinase, as well as tyrosyl-tRNA synthetase.
It mainly understood enzymes and possible classes for the enzyme chemistry.
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