Question

Select the incorrect statement w.r.t. Eco RI
O Its an endonuclease that breaks sugar-phosphate backbone of ds DNA
O Its an endonuclease that breaks phosphodiester bonds of a DNA molecule
Its an exonuclease that cleaves monophosphoester bonds
0 Its a nuclease that recognizes specific palindromic sequence​

Answer 1

Answer:

Eco RI is used to cut a segment of foreign DNA and that of a vector DNA to form a recombinant DNA.

• (i)The set of palindromic nucleotide sequence of base pairs the Eco RI-will

recognise in both the DNA segments. mark the site at which Eco RI will act and-cut both the segments.,

• (ii) Sticky ends formed on both the segments where the two DNA segments will join later to forma recombinant DNA.