Separated strands of DNA are prevented from coiling in replication
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Helicase unwinds the helix, and single-strand binding proteins prevent the helix from re-forming. Topoisomerase prevents the DNA from getting too tightly coiled ahead of the replication fork. DNA primase forms an RNA primer, and DNA polymerase extends the DNA strand from the RNA primer.
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The separated strands of DNA are prevented from coiling in replication by SSBP (single-strand binding proteins )
Explanation:
Replication is the process through which DNA duplicates and generates identical copies.
- Each daughter DNA molecule has an old and new strand after replication. The replication process is dubbed semi-conservative because parental DNA is partially conserved in each daughter's DNA.
- The nucleotides present in the nucleoplasm, are activated by ATP in the presence of an enzyme called phosphorylase during replication, and dATP (deoxyribonucleotide triphosphates)is formed
- The replication process starts at a certain point named 'O' and ends at a location called 'T.' Endonuclease, an enzyme, temporarily nicks one of the DNA strands at position 'O'. In the area of 'O,' the enzyme DNA helicase forms weak hydrogen bonds.
- The DNA strands unwind and split. The unwinding is bidirectional and continues as a replication fork in the shape of a 'Y'.Each strand that is split serves as a template.
SSBP (single-strand binding proteins) prevent the two split strands from recoiling.
- SSB proteins remain connected to both separated strands to facilitate the creation of new polynucleotide strands.
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