Question

separation of isolted dna​

Answer 1

Answer:

Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb(1). ... The phosphate backbone of the DNA (and RNA) molecule is negatively charged, therefore when placed in an electric field, DNA fragments will migrate to the positively charged anode.

Explanation:

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Answer 2

Answer:

ᴀɢᴀʀᴏsᴇ ɢᴇʟ ᴇʟᴇᴄᴛʀᴏᴘʜᴏʀᴇsɪs ɪs ᴛʜᴇ ᴍᴏsᴛ ᴇғғᴇᴄᴛɪᴠᴇ ᴡᴀʏ ᴏғ sᴇᴘᴀʀᴀᴛɪɴɢ ᴅɴᴀ ғʀᴀɢᴍᴇɴᴛs ᴏғ ᴠᴀʀʏɪɴɢ sɪᴢᴇs ʀᴀɴɢɪɴɢ ғʀᴏᴍ 100 ʙᴘ ᴛᴏ 25 ᴋʙ(1). ... ᴛʜᴇ ᴘʜᴏsᴘʜᴀᴛᴇ ʙᴀᴄᴋʙᴏɴᴇ ᴏғ ᴛʜᴇ ᴅɴᴀ (ᴀɴᴅ ʀɴᴀ) ᴍᴏʟᴇᴄᴜʟᴇ ɪs ɴᴇɢᴀᴛɪᴠᴇʟʏ ᴄʜᴀʀɢᴇᴅ, ᴛʜᴇʀᴇғᴏʀᴇ ᴡʜᴇɴ ᴘʟᴀᴄᴇᴅ ɪɴ ᴀɴ ᴇʟᴇᴄᴛʀɪᴄ ғɪᴇʟᴅ, ᴅɴᴀ ғʀᴀɢᴍᴇɴᴛs ᴡɪʟʟ ᴍɪɢʀᴀᴛᴇ ᴛᴏ ᴛʜᴇ ᴘᴏsɪᴛɪᴠᴇʟʏ ᴄʜᴀʀɢᴇᴅ ᴀɴᴏᴅᴇ.ᴀᴘʀ 

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