Question

Short answer on tissue culture

Answer 1

Plant tissue culture. Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation.

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Answer 2

Answer:

Tissue Culture:

The procedure in which a small tissue pieces or organs is removed from a donor plant and cultured aseptically on a nutrient medium is called Tissue Culture. This production is also known as micropropagation technique. The synthetic medium contains all the nutrients and hormones which are required for the growth.

A cell or tissue is transferred into suitable synthetic medium under sterile conditions. The tissue then develops into a fast growing cellular mass called callus. The callus is transferred to another medium for growth and differentiation that forms plantlets. The plantlets can be transplanted into soil or pots where they can be grown to maturity.

Tissue culture technique is being popularly used in agriculture and horticulture. It is used for production of ornamental plants like Orchids, Dahlia and Carnation. Other examples are Banana, Fig, Lemon, Potatoes, etc.

Advantages of tissue culture are:

1. A large number of clones can be produced from a single parent plant.
2. It enables us to select and culture the desirable characteristic traits.
3. It has short production time with long generation time.
4. Reproduction of plants is possible even in the absence of seeds or necessary external pollinators to produce seeds.
5. It frees us from the restrictions of growing plants only in certain seasons.
6. Endangered plant species on the verge of extinction can be saved with the tissue culture procedure.