short note on
2.Chromatography
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Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a system on which is fixed a material called the stationary phase.
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Chromatography
Chromatography is a physical method of separation that distributes components to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction.
- It is a technique used to separate mixtures in to their components, purify. Compounds which can be solid or liquid and also test the purity of compounds.
- In this technique the mixture of substances are applied on to a stationary phase .
- Then a pure solvent or a mixture of solvents is allowed to move slowly over stationary phase .The components of mixture gets gradually separated from one another .The moving phase is mobile phase .
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Additional information:-
The chromatography is classified into two categories:
- Adsorption chromatography
- Partition chromatography
Adsorption chromatography:
- It is based on the facts that different compounds are adsorbed on adsorbent at different degrees.
- The commonly used adsorbents are silica gel and alumina.
- When a mobile phase is allowed to move over a stationary phase the components of mixture move by varying distances over a stationary phase.
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