short note on DNA sequencing
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Answer:
Invitrogen Collibri whole-genome library prep kits improve library prep success for high-throughput Illumina™ next-generation sequencing (NGS) systems.
PCR-free protocols
Best coverage of challenging genomic regions with the physical shearing protocol
Improved sensitivity of variant detection from 1 ng of DNA compared to Illumina, Kapa Biosystems™, or NEB™ kits
Visual feedback for higher library prep success rates
Protocols available for DNA that is physically sheared (PS) or enzymatically sheared (ES)
Accommodates a wide range of sample types and inputs:
Intact DNA
Degraded DNA, including FFPE or cfDNA
Suitable for genomes of all sizes
Consistent gene coverage from 1 to 500–1,000 ng
Variant detection is consistent among low abundance (1 ng) and high abundance (500–1,000 ng) samples to confidently analyze variable samples.
Physically sheared DNA
Sequencing studies can now accept a greater diversity of samples and retain confidence in genomic interpretation. Variant detection rates by the Invitrogen Collibri PS DNA Library Prep Kits for Illumina Systems are similar between amplified (1–100 ng) libraries and PCR-free (500–1,000 ng) libraries.
Physically sheared DNA
Figure 1. Coriell NA12878 DNA was converted into a library and sequenced on an Illumina™ NovaSeq™ 6000 system at 372M reads per sample. SNP recall calculated using FreeBayes v1.2.0.
Explanation:
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Answer:
DNA polymer of nucleotide.