Question

Short notes on Immobilization of enzymes and it's application.

Answer 1

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An immobilized enzyme is an enzyme attached to an inert, insoluble material—such as calcium alginate. This can provide increased resistance to changes in conditions such as pH or temperature.

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Answer 2

Enzyme immobilization

Explanation:-

• Enzyme immobilization is a widely empirical technique to obtain more stable, active, and reusable enzymes.
• Empiricism can be mitigated by the application of rational design procedures employing bioinformatic tools, engineered proteins, and detailed analysis of existing data. Relevant approaches to rationalize the design of enzyme immobilization protocols, with a particular focus on the modulation of immobilization pH to regulate the operational stability of glutaraldehyde cross-linked enzymes and their integrity. Iron-rich backing is coated for durability. Iron-sensitive enzymes.
• Other strategies, such as the use of factorial planning, optimization of specific enzyme orientations through protein engineering, and the use of mathematical algorithms and in silico prediction tools have also been described to reduce classical empiricism.
• In conclusion, building a public repository has been proposed as a new promising tool to develop improvements in future rational design procedures of enzyme immobilization.

• Applications of immobilized enzymes:

• Stabilized enzyme system - should meet the requirements in terms of stability, activity, pH optimum, and other characteristics, all must be considered

, and other characteristics, all must be considered • Property of immobilized enzymes - the greatest industrial importance is that ease with which they can be separated from the reaction mixture

• Therefore, unlike systems consisting of soluble enzymes - the reaction can be stopped by physically removing the immobilized enzyme - without the need for processes such as heat inactivation that affect the products of the reaction

• Furthermore, the enzyme will remain active and largely uncontaminated, so it can be reused