Question

Significance of different ph in sds gel electrophoresis

Answer 1

Answer:

Control of the charge state of the glycine by the different buffers is the key to the whole stacking gel thing. So here's how the stacking gel works. When the power is turned on, the negatively-charged glycine ions in the pH 8.3 electrode buffer are forced to enter the stacking gel, where the pH is 6.8.

Answer 2

$\mathfrak{\large{\underline{\underline{Answer :}}}}$

The system is set up with a stacking gel at pH 6.8, buffered by Tris-HCl, a running gel buffered to pH 8.8 by Tris-HCl and an electrode buffer at pH 8.3