singel bhem spactometar
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Explanation:
In single beam spectrophotometers, a single beam of light passes through the sample holder. The instrument is standardized by placing a reference in the sample holder, and the resulting value is subtracted from subsequent sample measurements to remove effects from the solvent and the cell.
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A single beam spectrophotometer has only one beam of light, while a double beam spectrophotometer has two beams of light, one passing through a reference solution and one passing through the sample
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