Question

*Soil samples were collected from TNT-contaminated soil and placed in sterilized bags. Bacterial strains were isolated from the soil through serial dilution and subsequent culture in Petri dishes containing nutrient agar for 24–72 h at 30°C. Four microbial strains were identified: FA11, FA12, FA13 and FA14. PHA detection agar (PDA) medium* [Lee and Choi (1999): (NH4)2SO4, 2 g/L; KH2PO4, 13.3 g/L; MgSO4.7H2O, 1.3 g/L; citric acid, 1.7 g/L; agar, 15 g/L; glucose, 2 g/L; trace element solution, 10 mL/L (FeSO4.7H2O, 10 g/L; ZnSO4.7H2O, 2.25 g/L; CuSO4.5H2O,1 g/L; MnSO4.5H2O, 0.5 g/L; CaCl2.2H2O, 2.0 g/L; Na2B4O7 .10H2O, 0.23 g/L; NH4)6MO7O24, 0.1 g/L; HCl, 10 mL)] *was used for the qualitative screening of poly (3HB-co-3HV)-producing bacterial strains. The pH of the medium was adjusted to 7.0. Only strain FA11 was found to be capable of synthesizing poly(3HB-co-3HV) based on the presence of blue inclusion bodies and golden fluorescence as evidenced by Sudan black B and Nile blue A staining* Create a flowchart diagram of the given data to better illustrate the procedure, criteria and end result of the experiment

Answer 1

Answer:

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Explanation:

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