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Membrane Transporters in Drug Discovery and Development
Methods and Protocols
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Qing Yan
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Part of the Methods in Molecular Biology book series (MIMB, volume 637)
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Front Matter
Pages i-xiii
Membrane Transporters and Drug Development: Relevance to Pharmacogenomics, Nutrigenomics, Epigenetics, and Systems Biology
Qing Yan
Pages 1-21
Bioinformatics for Transporter Pharmacogenomics and Systems Biology: Data Integration and Modeling with UML
Qing Yan
Pages 23-45
Multidrug Resistance: Phylogenetic Characterization of Superfamilies of Secondary Carriers that Include Drug Exporters
Ming Ren Yen, Jonathan S. Chen, Jose L. Marquez, Eric I. Sun, Milton H. Saier
Pages 47-64
Targeting Drug Transporters – Combining In Silico and In Vitro Approaches to Predict In Vivo
Praveen M. Bahadduri, James E. Polli, Peter W. Swaan, Sean Ekins
Pages 65-103
Methods to Evaluate Transporter Activity in Cancer
Takeo Nakanishi, Douglas D. Ross, Keisuke Mitsuoka
Pages 105-120
Analysis of Expression of Drug Resistance-Linked ABC Transporters in Cancer Cells by Quantitative RT-PCR
Anna Maria Calcagno, Suresh V. Ambudkar
Pages 121-132
Fluorescence Studies of Drug Binding and Translocation by Membrane Transporters
Frances J. Sharom, Ronghua Liu, Balpreet Vinepal
Pages 133-148
A Model for Transport Studies of the Blood–Brain Barrier
Dennis J. Bobilya
Pages 149-163
Genetic Variants in the Vesicular Monoamine Transporter 1 (VMAT1/SLC18A1) and Neuropsychiatric Disorders
Falk W. Lohoff
Pages 165-180
Equilibrium Binding and Transport by Vesicular Acetylcholine Transporter
Parul Khare, Aubrey R. White, Anuprao Mulakaluri, Stanley M. Parsons
Pages 181-219
ABC Transporters in Ophthalmic Disease
Corey Westerfeld
Pages 221-230
Imaging of Protein Translocation In Situ in Skeletal Muscle of Living Mice
Hans P.M. M. Lauritzen
Pages 231-244
Glucose Transporters in Parasitic Protozoa
Scott M. Landfear
Pages 245-262
NMR Studies of Membrane Proteins
Gabriel A. Cook, Stanley J. Opella
Pages 263-275
Site-Directed Mutagenesis in the Study of Membrane Transporters
Audra A. McKinzie, Renae M. Ryan, Robert J. Vandenberg
Pages 277-293
Xenopus laevis Oocytes
Stefan Bröer
Pages 295-310
Measurement of Intracellular pH
Frederick B. Loiselle, Joseph R. Casey
Pages 311-331
Measurement of Plasma Membrane Calcium–Calmodulin-Dependent ATPase (PMCA) Activity
Tamer M. A. Mohamed, Florence M. Baudoin-Stanley, Riham Abou-Leisa, Elizabeth Cartwright, Ludwig Neyses, Delvac Oceandy
Pages 333-342
Assessment of the Contribution of the Plasma Membrane Calcium ATPase, PMCA, Calcium Transporter to Synapse Function Using Patch Clamp Electrophysiology and Fast Calcium Imaging
Chris J. Roome, Ruth M. Empson
Pages 343-360
Back Matter
Pages 361-365
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