Solid phase automated synthesis of dna biology discussion
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In solid-phase synthesis, an oligonucleotide being assembled is covalently bound, via its 3'-terminal hydroxy group, to a solid support material and remains attached to it over the entire course of the chain assembly.
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Solid-phase synthesis has a number of advantages over solution synthesis: large excesses of solution-phase reagents can be used to drive reactions quickly to completion. impurities and excess reagents are washed away and no purification is required after each step.
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