Some of the parameters considered for change evaluation are
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Evaluation Parameters: TRB's National Cooperative Highway Research Program (NCHRP) Report 738: ...
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You are asked to perform multiple sequence alignment of certain dna region of 10 organisms with clustalw using a fixed scoring scheme. The rate of substitutions of that region of dna across 10 different organisms of length 1000 bases is estimated as 10-9 per base per year. Considering that the group of organism diverged approximately 10 million years ago
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