Soumya was purifying an enzyme from a homogenate of muscle cells. She went through
seven steps of purification and found that the enzyme activity was the same as the
homogenate value. On the eighth step, when the protein was highly pure, the enzyme
activity rose to five times that of the homogenate value. Can you suggest a possible reason?
a. The homogenate assays are wrong
b. Step eight has co-purified an activator of the enzyme
c. The enzyme has an inhibitor present in the muscle cell homogenate
d. The temperature at step eight was just right for the enzyme activity
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