Question

Spillover spread matrix and fluorescence spillover to maximize resolution

Answer 1

After compensation, the measurement errors arising from multiple fluorescences spilling into each detector become evident by the spreading of nominally negative distributions. Depending on the instrument configuration and performance, and reagents used, this “spillover spreading” affects sensitivity in any given parameter. The degree of spillover spreading had been predicted theoretically to increase with measurement error, i.e., by the square root of fluorescence intensity, as well asdirectly related to the spectral overlap matrix coefficients.