Question

spread plate method explain step by step ​

Answer 1

Procedure of Spread Plate Technique

Make a dilution series from a sample.

• Pipette out 0.1 ml from the appropriate desired dilution series onto the center of the surface of an agar plate.
• Dip the L-shaped glass spreader into alcohol.
• Flame the glass spreader (hockey stick) over a Bunsen burner.

Answer 2

Answer:

The spread plate technique involves using a sterilized spreader with a smooth surface made of metal or glass to apply a small amount of bacteria suspended in a solution over a plate. The plate needs to be dry and at room temperature so that the agar can absorb the bacteria more readily

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