Question

stain used to observe nucleus in onion peel______?​

Answer 1

Answer:

Iodine

Explanation:

Iodine is used to let the onion peel skin show in general and can also help show the other parts in it.

Answer 2

The stain used to observe the nucleus in the onion peel is iodine solution or methylene blue.

How to prepare a temporary stained slide of an onion bulb peel?

Requirements:

Onion bulb, glass slide, glycerine, watch glass, coverslip, forceps, scalpels, needles, iodine solution or methylene blue, microscope, etc.

Procedure :

1. Separate a thin onion scale from an onion.

2. Tear it from the concave side to get a transparent, thin, and membranous onion peel piece called the epidermis.

3. Now, keep this onion peel piece in a watch glass containing water.

4. Cut out a small portion of this peel and place it flat on a glass slide on a drop of water with the help of a thin camel-hair paint brush.

5. Add a drop of an iodine solution or methylene blue stain.

6. Drain the excess stain and mount the onion peel in a drop of glycerine under a coverslip.

7. Examine the slide under a compound microscope's low and high powers.

Observations :

• The epidermal parenchymal onion peel cells can be seen as linear or rectangular compartments with rigid cell walls.
• The nucleus is pushed towards the periphery due to the presence of a central vacuole.

Precautions:

1. Immediately put the peel of the onion bulb in water containing petri-dish to avoid it's folding and drying.

2. Spread the peel uniformly on the slide.

3. Excess stain should be drained off.

4. There should be no air bubbles under the coverslip.

Similar observations are found in the peel taken from the undersurface of a leaf of Tradescantia or Rhoe or temporary mounts of peels of onions of different sizes.

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