Question

Staining of specimen for observing under microscope-steps.

Answer 1

Place a drop of fluid in the center of the slide.

Position sample on liquid, using tweezers.

At an angle, place one side of the cover slip against the slide making contact with outer edge of the liquid drop.

Lower the cover slowly, avoiding air bubbles.

Remove excess water with the paper towel.

Step 1: Crystal Violet. primary stain added to the specimen smear. ...

Step 2: Iodine. mordant, makes the dye less soluble so it adheres to cell walls. ...

Step 3: Alcohol. the decolorizer, washes away stain from gram-negative cell walls. ...

Step 4: Safranin. counterstain allows dye adherence to gram-negative cells.