Question

State (step wise) all the process of CLONING.

Answer 1

Cloning is the process of producing genetically identical individuals of an organism either naturally or artificially. In nature, many organisms produce clones through asexual reproduction. Cloning in biotechnology refers to the process of creating clones of organisms or copies of cells or DNA fragments. 

Answer 2

Gene Cloning Step # 1.

Isolation of DNA (Gene of Inter­est) Fragments to be Cloned

Gene Cloning Step # 2.

Insertion of Isolated DNA into the a Suitable Vector to Form the Recombinant DNA

Gene Cloning Step # 3.

Introduction of the Recombinant DNA into a Suitable Organism known as Host

Gene Cloning Step # 4.

Selection of the Transformed Host Cells and Identification of the Clone Con­taining the Gene of Interest

Gene Cloning Step # 5.

Multiplication/Expression of the Introduced Gene in the Host

Gene Cloning Step # 6.

Isolation of the Multiplied Gene Copies/Protein Expressed by the Intro­duced Gene

Gene Cloning Step # 7.

Purification of the Isolated Gene Copy/Protein

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