state the principle used in separation by centrifugation and chromatography
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Centrifugation is based on the difference in the density of the particles of the components of a mixture. The principle is that the denser particles are forced to the bottom and the lighter particles stay at the top when spun rapidly.
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The principle is that the denser particles are forced to the bottom and the lighter particles stay at the top when spun rapidly. The apparatus used for centrifugation is called a centrifuge. ... When the rotation stops, the solid particles end up at the bottom of the centrifuge tube with liquid at the top.
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Chromatography is a non-destructive procedure for resolving a complex mixture into its individual fractions or compounds. This is a separation procedure and the separated entities are identified by other analytical techniques like UV-visible, Infrared, NMR (nuclear magnetic resonance), Mass spectroscopy etc. For quantitative analysis the measurement of the area under the curve in chromatogram is done.
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