state three principle of chromatography.
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Explanation:
Principle of chromatography :
• chromatography is used to separate complex mixture
• example :- kerosene oil from water
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Principles of chromatography
Chromatography is based on the principle of separation of compounds into different bands (color graphs) and the identification of those bands.
The preferential separation is done due to differential affinities of compounds towards stationary and mobile phase. After separation of the compounds, they are identified by suitable detection methods.
The differences in affinities arise due to relative adsorption or partition coefficient in between components towards both phases.
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