Question

Steps involved in tissue culture

Answer 1

Steps in plant tissue culture

STAGE 1: Initiation phase. The initiation phase is the first phase of tissue culture.

STAGE 2: Multiplication stage.

STAGE 3: Root formation.

Medium preparation:

Plant preparation:

Transferring the plant material to a tissue culture medium:

Technique for Plant in Vitro Culture

Answer 2

Main steps in Tissue Culture are:-

1. Selection of plant
2. Isolation of explant
3. Sterilization of explant
4. Inoculation of explant
5. Incubation
6. Initiation of callus
7. Sub-culturing
8. Regeneration
9. Hardening