Question

steps of bacterial transformation???

Answer 1

Answer:

Key steps in the process of bacterial transformation: (1) competent cell preparation, (2) transformation of cells, (3) cell recovery, and (4) cell plating.

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Answer 2

Answer:

• Take competent cells
• Add foreign DNA
• Recombinant DNA can then be forced into such cells by incubating the cells with recombinant DNA on ice, followed by placing them briefly at 42°C (heat shock) and then putting them back on ice.
• This enables the bacteria to take up recombinant DNA.