Steps of preparation of temporary mount of a leaf peel to observe stomata are
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The steps are as follows -
1. First remove the stomatal covering of the leaf carefully.
2. Then, colour it by adding saffranin for better observation.
3. Clean leaf peel if extra amount of saffranin is added.
4. Then keep it on a glass slide and add few drops of glycerine to it.
5. Finally, cover it with a cover-slip.
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1 removal of leaf peel from the bottom surface.
2 putting it in a water.
3 after removing from water staining it with saffranin.
4 put it in a clean slide and mount with glycerine with the help of a brush.
5 put another slide gently over it.
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