steps of testing a leaf for starch
Answers
Answer:
Put a few drops of iodine solution on the leaf if starch is present the brown hiding solution will turn blue black .
(Hopes this helps )
៚Starch Testing :
☆ Iodine solution is used to test leaves for the presence of starch. You need to:
- Heat a plant leaf in boiling water for 30 seconds (this stops its chemical reactions).
- Heat it in boiling ethanol for a few minutes (this removes most of its colour).
- Wash with water and spread onto a white tile.
- Add iodine solution from a dropping pipette.
➢ After a few minutes, the parts of the leaf that contain starch turn blue-black.
➢ Note that ethanol is heated using a hot water bath. Ethanol boils at 78°C, so a tube of it boils when placed in a beaker of hot water. This is safer than using a Bunsen burner because ethanol is flammable.
➢ Variegated leaves have green parts (where the cells contain chlorophyll) and white parts (where there is no chlorophyll). Only the parts that were green become blue-black with iodine solution, showing the importance of chlorophyll in photosynthesis.
➢ A plant can be ‘de-starched’ by leaving it in the dark for a few hours. Parts of its leaves are covered with dark paper, and the plant is left in the light for a few hours. Only the uncovered parts become blue-black with iodine solution, showing the importance of light in photosynthesis.
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