Question

sterilization of plant and animal tissue culture media

Answer 1

Plant tissue culture media are generally sterilized by autoclaving at 121 °C and 1.05 kg/cm2 (15-20 psi). The time required for sterilization depends upon the volume of medium in the vessel.

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Answer 2

The plant and animal tissue culture media is sterilized by autoclaving at 120°C for 15 minutes to kill germs, moist heat or condensed hot air is employed in this procedure.

Explanation:-

1. Sterilization is a procedure that is primarily used to destroy all types of microorganisms and their spores.
2. This procedure is utilized to maintain a clean atmosphere.
3. It is commonly accomplished by combining numerous elements such as heat, irradiation, filtration, high pressure, etc.
4. Sterilization treatments are aimed to destroy or eliminate a wide variety of microorganisms, including protozoa, fungus, bacteria, and viruses.
5. Most cell and organ culture materials are sterilized by autoclaving, dry heat sterilization, and filtration.