Question

strategy used to avoid multiple insert ligation and self ligation of vector?​

Answer 1

THE MOST BASIC STEP FOR PREVENTION OF SELF LIGATION IS CUTTING THE INSERT AND VECTOR WITH 2 DIFFERENT RESTRICTION ENZYMES, GENERATING FRAGMENTS WITH 2 DIFFERENT RESTRICTION SITES. Removing 5'-phosphate groups from the vectors using phosphatases (e.g. alkaline phosphatase), prevents self- ligation. dephosphorylated.

Answer 2

Answer:

THE MOST BASIC STEP FOR PREVENTION OF SELF LIGATION IS CUTTING THE INSERT AND VECTOR WITH 2 DIFFERENT RESTRICTION ENZYMES, GENERATING FRAGMENTS WITH 2 DIFFERENT RESTRICTION SITES. Removing 5'-phosphate groups from the vectors using phosphatases (e.g. alkaline phosphatase), prevents self- ligation. dephosphorylated.

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