Question

Structure of endoplasmic reticulum can be isolated

Answer 1

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↪️↪️↪️↪️Plant cells contain a well-defined endoplasmic reticulum (ER), which like its mammalian cell counterpart has several characteristic functions, including protein synthesis, glycosylation, and lipid synthesis.

↪️↪️↪️These functions account, in part, for the observation that the cytoplasmic surface of the ER is predominantly studded with ribosomes (rough ER).

↪️↪️↪️↪️ The ER is a complex, convoluted network of interconnecting membrane channels and sheets, and is inevitably damaged during even the gentlest and briefest cellular homogenization.

↪️↪️↪️↪️↪️↪️As a result, the ER is conveniently fragmented into a large number of small closed vesicles called “microsomes” that can be readily isolated by differential or density gradient centrifugation.

↪️↪️↪️↪️↪️The chapter describes methods and assays that have been employed to isolate and characterize ER membranes from the germinating castor bean endosperm tissue

.↪️↪️↪️↪️↪️↪️↪️ The active sites of several phospholipid-synthesizing enzymes have been localized to the cytoplasmic surface of the ER. Other major membrane phospholipids, such as phosphatidylcholine and .
↪️⭐⭐phosphatidylethanolamine are synthesized by enzymes present in the ER membrane.