Sudan Black B' stain is used to stain (a) nucleic acid (b) protein
Answers
Sudan Black B (C29H24N6) is a nonfluorescent, relatively thermostable lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. It has the appearance of a dark brown to black powder with maximum absorption at 596–605 nm and melting point 120–124 °C. It stains blue-black.
Sudan Black B is one of the dyes used for Sudan staining. Similar dyes include Oil Red O, Sudan III, and Sudan IV.
Sudan Black B can be used to stain some other materials than the other Sudan dyes, as it is not so specific to lipids.
A use of Sudan Black B is in fingerprint enhancement. It is useful for detecting fats that are contaminated with oil and grease.
In differentiating haematological disorders Sudan black will stain myeloblasts but not lymphoblasts.
Sudan Black B was introduced by Lison and Dagnelie in 1935.