Sumitha added ten drops of copper sulphate solution and two drops of sodium hydroxide
solution to a sample of egg white in water. What change did she observe in the
sample? What did she infer from this activity?
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When two drops of copper sulphate and ten drops of caustic soda or NaOH is added to egg, it turns violet. This indicates the presence of proteins.
This is Biuret's test and the principle is simple as follows:
In the presence of alkaline medium (caustic soda or NaOH), the Cu2+ ions get reduced to Cu+ and binds to the egg proteins, thereby giving a violet color.
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