sunflower seeds contain a nutrient which is broken down by lipase during germination. which test would detect this nutrient?
Answers
Lipase enzymes have become more and more prominent on the enzyme biotechnology scenario due to their versatility for hydrolysis and synthesis, their catalytic reactions often being chemo-selective, region-selective or enantio-selective. Lipases are used in many sectors such as the food, pharmaceutical, fine chemical, oil chemical, biodiesel and industrial detergent industries (Freire and Castilho 2008, Alonso et al. 2005). The participation of lipases in the worldwide enzyme industry market has grown significantly and it is believed that, in the future, they will acquire importance comparable to that of the peptidases, which currently represent 25 to 40% of industrial enzyme sales (Hasan et al. 2006).
Lipases act, by definition, at the organic-aqueous interface, catalyzing the hydrolysis of ester-carboxylate bonds and releasing fatty acids and organic alcohols (Pereira et al., 2003; Leal et al. 2002; Kamimura et al., 1999; Merçon et al. 1997). However, as Pottevin showed for the first time in 1906, in water-restricted environments, the reverse reaction (esterification) or even various transesterification reactions can occur (Freire and Castilho 2008, Castro et al. 2000). The term transesterification refers to the exchange of groups between an ester and an acid (acidolysis), between an ester and an alcohol (alcoholysis) or between two esters (interesterification).
I'm not sure but maybe you use ethanol to detect the presence of fats..I don't think it's any named test but their are several other test we can use to detect the presence of lipid or fats in sunflower seed.